1. Correction of dissolved oxygen and pH electrodes.
2. Tank sterilization. Medium into the tank as needed, as required to seal the tank add large autoclaves and sterilization.
3. The tank is cooled, place it in the fermentation stage, install good; open cooling water, power up the pump and start connecting ventilation pipe ventilation, adjustthe air inlet knob ventilation suitable power on the fermentation tanks, settings, such as temperature, pH, agitation speed, 640r/min openTurning 30min, dissolved oxygen electrode setting is 100.
4. Temperature stability, parameters are correct, the rocking good seed access, start fermentation time, and began recording various parameters.5, fermentation is finished cleaning tank and electrodes, electrodes inserted in flask 4 m KCL for later use.6, as required, fill out the registration, signed by the head.