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Fermentor (Bioreactor): History

Jul 22, 2019

Meaning of Fermentor:

A fermentor (bioreactor) is a closed vessel with adequate arrangement for aeration, agitation, temperature and pH control, and drain or overflow vent to remove the waste biomass of cultured microorganisms along-with their products.

A fermentor is used for commercial production in fermentation industries and is a device in which a substrate of low value is utilized by living cells or enzymes to generate a product of higher value. Fermentors are extensively used for food processing, fermentation, waste treatment, etc.

bioreactor-50L

History of Fermentors:

De Beeze and Liebmann (1944) used the first large scale (above 20 litre capacity) fermentor for the production of yeast. But it was during the first world war, a British scientist named Chain Weizmann (1914-1918) developed a fermentor for the production of acetone.

Since importance of aseptic conditions was recognised, hence steps were taken to design-and construct piping, joints and valves in which sterile conditions could be achieved and manufactured when required.

For the first time, large scale aerobic fermentors were used in central Europe in the year 1930’s for the production of compressed yeast (de Becze and Leibmann, 1944). The fermentor consisted of a large cylindrical tank with air introduced at the base via network of perforated pipes.

In later modifications, mechanical impellers were used to increase the rate of mixing and to break up and disperse the air bubbles. This process led to the compressed air requirements. Baffles on the walls of the vessels prevented forming a vortex in the liquid. In the year 1934, Strauch and Schmidt patented a system in which the aeration tubes were introduced with water and steam for cleaning and sterilization.

The decision to use submerged culture technique for penicillin production, where aseptic conditions, good aeration and agitation were essential, was probably a very important factor in forcing the development of carefully designed and purpose-built fermentation vessels.

In 1943, when the British Govt. decided that surface culture was inadequate, none of the fermentation plants were immediately suitable for deep fermentation. The first pilot fermentor was erected in India at Hindustan Antibiotic Ltd., Pimpri, Pune in the year 1950.

Design of Fermentors:

All bioreactors deal with heterogeneous systems dealing with two or more phases, e.g., liquid, gas, solid. Therefore, optimal conditions for fermentation necessitate efficient transfer of mass, heat and momentum from one phase to the other. Chemical engineering principles are employed for design and operation of bioreactors.

A bioreactor should provide for the following:

(i) Agitation (for mixing of cells and medium),

(ii) Aeration (aerobic fermentors); for O2 supply,

(iii) Regulation of factors like temperature, pH, pressure, aeration, nutrient feeding, liquid level etc.,

(iv) Sterilization and maintenance of sterility, and

(v) Withdrawal of cells/medium (for continuous fermentors).

Modern fermentors are usually integrated with computers for efficient process monitoring, data acquisition, etc.

Generally, 20-25% of fermentor volume is left unfilled with medium as “head space” to allow for splashing, foaming and aeration. The fermentor design varies greatly depending on the type and the fermentation for which it is used. Bioreactors are so designed that they provide the best possible growth and biosynthesis for industrially important cultures and allow ease of manipulation for all operations.

Size of Fermentors:

The size of fermentors ranges from 1-2 litre laboratory fementors to 5,00,000 litre or, occasionally, even more, fermentors of upto 1.2 million litres have been used. The size of the fermentor used depends on the process and how it is operated. A summary of fermentor or size of fermentor (litres) Industrial product sizes for some common microbial fermentations is given in Table 39.6.

 Fermentor (Bioreactor)

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